Discovery of a novel polymer for xeno-free, long-term culture of human pluripotent stem cell expansion

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Publication date
2021-01-20
Creators
Nasir, Aishah
Thorpe, Jordan
Burroughs, Laurence
Alexander, Morgan
Denning, Chris
Meurs, Joris
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Description
Discovery of poly(TCDMDA-blend-BA) polymer substrate offers a cost-effective and xeno-free adherent culture system for human pluripotent stem cells (hPSCs) in E8 medium. This is a scalable substrate which can be readily applied to common tissue culture plasticware (TCP), whilst retaining stem cell integrity after long-term serial passage. Fulfillment of all regulatory cell culture requirements makes this a desirable expansion platform for clinical-scale, therapeutic and biomedical applications. Data presented herein includes fluorescence microscopy of high-throughput attachment screening studies.
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Subjects
  • Stem cells
  • Cell culture
  • Biomedical materials
  • Polymer microarray, Biomaterials discovery, Essential 8 medium, Xeno-free, human pluripotent stem cells
  • Engineering::General engineering::Bioengineering, biomedical engineering & clinical engineering::Biomaterials
  • Q Science::QH Natural history. Biology::QH573 Cytology
Divisions
  • University of Nottingham, UK Campus::Faculty of Medicine and Health Sciences::School of Medicine
Research institutes and centres
  • University of Nottingham, UK Campus::Biomolecular Sciences, Centre for
Deposit date
2021-01-20
Data type
cell microscopy, Phosphokinase array, protein adsorption surface analysis, growth curves cell analysis, integrin blocking assays
Funders
  • Engineering & Physical Sciences Research Council
  • Wellcome Trust
  • British Heart Foundation
  • National Centre for the Replacement, Refinement, and Reduction of Animals in Research (NC3Rs)
Grant number
  • EP/N006615/1
Parent project
  • 'Future-Proof' Synthetic Surfaces for the Automated Manufacture of Human Pluripotent Stem Cells (EP/H045384/1)
Collection dates
  • 01/12/15 - 01/08/19
Data collection method
Cell microscopy (IMSTAR), Operetta (Perkin Elmer), Phosphokinase R&D kit, liquid extraction surface analysis-tandem mass spectrometry (LESA-MS/MS; TriVersa Nanomate- Advion Biosciences), Cell analysis with Cedex HiRes Analyzer-the Roche Cell Counter
Legal and ethical issues
A patent has been filed in relation to the work described herein.
Provenance / lineage
https://onlinelibrary.wiley.com/doi/full/10.1002/adma.201501351
Resource languages
  • en_US
Copyright
  • University of Nottingham
Publisher
The University of Nottingham
CC-BY
Except where otherwise noted, this item's license is described as Creative Commons by Attribution