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      An in vivo systematic genetic analysis of tumour progression in Drosophila - RNAi line: 12399R-1

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      Publication date
      2019-05-01
      Creators
      Georgiou, Marios
      Metadata
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      Description
      Metastasis is the leading cause of death for cancer patients. Consequently it is imperative that we improve our understanding of the molecular mechanisms that underlie progression of tumour growth towards malignancy. Advances in genome characterisation technologies have been very successful in identifying commonly mutated or misregulated genes in a variety of human cancers. A major challenge however is the translation of these findings to new biological insight due to the difficulty in evaluating whether these candidate genes drive tumour progression. Using the genetic amenability of Drosophila melanogaster we generated tumours with specific genotypes in the living animal and carried out a detailed systematic loss-of-function analysis to identify numerous conserved genes that enhance or suppress epithelial tumour progression. This enabled the discovery of functional cooperative regulators of invasion and the establishment of a network of conserved ‘invasion suppressors’. RNAi line: 12399R-1 (III) Source: NIG Name: Mad (1) Full name: Mothers against dpp Also known as: pMad, dMad Annotation symbol: CG12399 FlyBase ID: FBgn0011648 File naming convention: File names typically contain representations of date (DDMMYY), RNAi Line, Animal Number and, in some cases, window (to accommodate larger samples that require multiple image stacks)
      External URI
      • https://rdmc.nottingham.ac.uk/handle/internal/6675
      DOI
      • http://doi.org/10.17639/nott.6668
      Links
      • http://flycancerscreen.nottingham.ac.uk
      • http://flybase.org/reports/FBgn0011648
      • http://doi.org/10.17639/nott.6668
      Subjects
      • Metastasis
      • Cancer -- Molecular aspects
      • Cancer -- Genetic aspects
      • Neoplasm Metastasis
      • Molecular Biology
      • Neoplasms -- genetics
      • Drosophila, cancer, invasion, metastasis, screen, RNAi, lgl, tumour suppressor, invasion suppressor
      • Biological Sciences::Zoology::Cell zoology
      • Biological Sciences::Biology::Cell biology
      • Biological Sciences::Genetics::Medical & veterinary genetics
      • Q Science::QL Zoology::QL360 Invertebrates
      • Q Science::QH Natural history. Biology::QH426 Genetics
      • Q Science::QH Natural history. Biology::QH201 Microscopy
      • R Medicine::RC Internal medicine::RC 254 Neoplasms. Tumors. Oncology (including Cancer)
      Divisions
      • University of Nottingham, UK Campus::Faculty of Medicine and Health Sciences::School of Life Sciences
      Deposit date
      2019-05-10
      Data type
      Image files
      Contributors
      • Canales Coutino, Brenda
      • Cornhill, Zoe E.
      • Couto, Africa
      • Mack, Natalie A.
      • Rusu, Alexandra D.
      • Nagarajan, Usha
      • Fan, Yuen Ngan
      • Hadjicharalambous, Marina R.
      • Lourdusamy, Anbarasu
      • Castellanos Uribe, Marcos
      • May, Sean T.
      • Rahman, Ruman
      Funders
      • Cancer Research UK
      Grant number
      • C36430, A12891
      Collection dates
      • October 2012 - April 2017
      Data collection method
      Live imaging was performed with either a Leica SP2 inverted confocal microscope equipped with a × 40/1.25 NA oil objective with PL APO correction, a Zeiss LSM880 inverted confocal microscope equipped with a x 40/ 1.30 NA oil Ph3 M27 objective or a Zeiss LSM5 Exciter AxioObserver equipped with an EC Plan-NeoFluar × 40/1.30 oil lens. Z-series were acquired using 1 μm z-sectioning.
      Resource languages
      • en
      Additional information
      This data set forms part of the larger 'Fly Cancer Screen' data set, which consists of over 700 image sets
      Publisher
      University of Nottingham

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