Discovery of a novel polymer for human pluripotent stem cell expansion and multilineage differentiation - Supporting data 1
Description
A scalable and cost-effective synthetic polymer substrate that supports robust expansion and subsequent multilineage differentiation of human pluripotent stem cells (hPSCs) with defined commercial media is presented. This substrate can be applied to common cultureware and used off-the-shelf after long-term storage. Expansion and differentiation of hPSCs are performed entirely on the polymeric surface, enabling the clinical potential of hPSC-derived cells to be realized.
Data presented herein includes time-of-flight secondary-ion mass spectrometry (ToF-SIMS), X-ray photoelectron spectroscopy (XPS), water contact angle (WCA) measurements, fluorescence microscopy, cell electrophysiology, nuclear magnetic resonance (NMR) spectroscopy, gel permeation chromatography (GPC).
Please note that this data set is in two parts, the second part of which can be found here: http://doi.org/10.17639/nott.16
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Subjects
- Polymers in medicine
- Stem cells
- Human cell culture
- Cell differentiation
- Stem Cells
- Cell Culture Techniques
- Cell Differentiation
- Polymers
- high throughput; human pluripotent stem cells; materials discovery; polymer microarrays; stem cell differentiation; ToF-SIMS; XPS; WCA; Cell Microscopy; Electrophysiology; NMR and GPC datasets.
- JACS Subjects::Medicine and Dentistry::Clinical medicine
- JACS Subjects::Biological Sciences::Molecular biology, biophysics & biochemistry
- JACS Subjects::Biological Sciences::Biology::Cell biology
- JACS Subjects::Physical sciences::Chemistry::Analytical chemistry
- JACS Subjects::Physical sciences::Chemistry::Organic chemistry::Polymer chemistry
- JACS Subjects::Physical sciences::Materials science
- JACS Subjects::Engineering::Chemical, process & energy engineering::Chemical engineering::Biochemical engineering
- Library of Congress Subject Areas::T Technology::TP Chemical technology::TP1080 Polymers and polymer manufacture
- Library of Congress Subject Areas::R Medicine::R Medicine (General)::R855 Medical technology. Biomedical engineering. Electronics
Divisions
- Faculties, Schools and Departments::University of Nottingham, UK Campus::Faculty of Medicine and Health Sciences::School of Medicine
Research institutes and centres
- Research institutes and centres::University of Nottingham, UK Campus::Biomolecular Sciences, Centre for
Deposit date
2015-06-04Data type
Raw data files for ToF-SIMS, XPS, WCA, Cell Microscopy, Electrophysiology, NMR and GPC datasets.Funders
- Funders::Engineering & Physical Sciences Research Council
- Funders::Wellcome Trust
- Funders::Biotechnology & biological Sciences Research Council
- Funders::Medical Research Council
- UK Regenerative Medicine Platform
- British Heart Foundation
- Heart Research UK
- National Centre for the Replacement, Refinement and Reduction of Animals in Research
Grant number
- EP/H045384/1
Parent project
- 'Future-Proof' Synthetic Surfaces for the Automated Manufacture of Human Pluripotent Stem Cells (EP/H045384/1)
Collection dates
- 01/10/2010 - 01/06/2015
Data collection method
ToF-SIMS (SurfaceLab - ION-TOF), XPS (CASA XPS), WCA, Cell Microscopy (IMSTAR), Electrophysiology, NMR (BRUKER) and GPC (Shimadzu) datasets.Legal and ethical issues
A patent has been filed in relation to the work described herein.Provenance / lineage
See methods in manuscript (DOI: 10.1002/adma.201501351)Resource languages
- en
Copyright
- University of Nottingham