Heat and salinity stress on the African eggplant

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Publication date
2023-11-01
Creators
David-Rogeat, Noémie
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Description
This data was collected as part of the research on the heat and salinity stress on the African eggplant to describe the effects of the stress combination on this crop. The data was collected at East Malling, UK, and comprises harvest data, physiological measurements, and nutritional quality data.
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Subjects
  • Eggplant -- Effect of heat on
  • Eggplant -- Effect of stress on
  • Eggplant -- Effect of salts on
  • Solanum aethiopicum, heat, salinity, stomatal conductance, nutrients
  • Biological Sciences::Botany::Plant physiology
  • Q Science::QK Botany::QK710 Plant physiology
Divisions
  • University of Nottingham, UK Campus::Faculty of Science::School of Biosciences
Deposit date
2023-10-20
Data type
Continuous and count data
Contributors
  • Broadley, Martin
  • Stavridou, Eleftheria
Funders
  • Biotechnology & biological Sciences Research Council
Data collection method
Leaf expansion rate was collected 3 times a week by measuring the length and maximum width of two leaves. SPAD, φ2, and φNPQ were measured three times a week 5 h after the start of the photoperiod using a MultispeQ V2.0 device with Photosynthesis RIDES 2.0 protocol. Leaf chlorophyll, flavonoids, and nitrogen balance were measured three times a week at the same time using a Dualex® device (ForceA). Stomatal conductance was recorded 4 h after the start of the photoperiod using a leaf porometer (Model SC-1, Decagon Devices, METER group, Pullman, WA, United States). Diurnal stomatal conductance measurements were taken at 0, 4, 8, and 12 h after the start of the photoperiod. On the last day of the experiment, a leaf sub-sample representing a mix of developing and developed leaves was removed from the plant, placed in liquid nitrogen straight after recording its combined weight, and stored at -80°C for biochemical analysis. The rest of the leaves and stems were weighed separately, after measuring leaf number, stem diameter and plant height, and oven-dried at 80°C until reaching a constant weight. Leaf electrolyte leakage was recorded on the last day of the experiment by placing 5 leaf discs in distilled water and recording the electrical conductivity (EC1) of the solution 24 h after collection using a LAQUAtwin EC-33 meter (Horiba, Kyoto, Japan). A second measure (EC2) was taken after the discs were boiled for 60 min and EL = EC1/EC2. Soil electrical conductivity was measured by placing 5 g of air-fried soil in 25 g of distilled water and recording the electrical conductivity of the solution after 24 h. Leaf chlorophyll content was measured by placing 2 lif discs in 10 ml of cold ethanol and measuring the absorbance of the supernatant after 48 h (Ultrospe III, Pharmaci LKB). Calculation were based on the method described by Wintermans and De mots (1965). Total carbohydrates were measured following the method of Dubois et al. (1956). Total antioxidants were measured following the TEAC protocol of Re et al. (1999) and total phenols were measured following the method of Singleton et al. (1999). Leaf nutrient concentrations were measured in an ICP-MS following an acid digestion (Thermo-Fischer Scientifi iCAP-Q). A FlashEA 1112 elemental analyser was used for leaf nitrogen content.
Resource languages
  • en
Publisher
The University of Nottingham
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Except where otherwise noted, this item's license is described as Creative Commons by Attribution