An in vivo systematic genetic analysis of tumour progression in Drosophila - RNAi line: 1451R-1

Metastasis is the leading cause of death for cancer patients. Consequently it is imperative that we improve our understanding of the molecular mechanisms that underlie progression of tumour growth towards malignancy. Advances in genome characterisation technologies have been very successful in identifying commonly mutated or misregulated genes in a variety of human cancers. A major challenge however is the translation of these findings to new biological insight due to the difficulty in evaluating whether these candidate genes drive tumour progression. Using the genetic amenability of Drosophila melanogaster we generated tumours with specific genotypes in the living animal and carried out a detailed systematic loss-of-function analysis to identify numerous conserved genes that enhance or suppress epithelial tumour progression. This enabled the discovery of functional cooperative regulators of invasion and the establishment of a network of conserved ‘invasion suppressors’.

RNAi line: 1451R-1 (III)
Source: NIG

Name: APC (2)
Full name: APC-like
Also known as: APC1, D-APC, dAPC, dAPC1
Annotation symbol: CG1451
FlyBase ID: FBgn0015589

File naming convention: File names typically contain representations of date (DDMMYY), RNAi Line, Animal Number and, in some cases, window (to accommodate larger samples that require multiple image stacks)

Included files: 060813_Lgl1451R1An4w1_combined.tif 060813_Lgl1451R1An4w2_combined.tif 070813_An1_Lgl_1451R1w_combined.tif 170713_An6_Lgl_1451R1_w_combined.tif 180613_Lgl1451R1An3w1_combined.tif 180613_Lgl1451R1An3w2_combined.tif 180613_Lgl1451R1An5w1_combined.tif 180613_Lgl1451R1An5w2_combined.tif 200613_lgl_1451r1_combined.tif 200813_1451R1_A4_W1_combined.tif 200813_1451R1_A4_W2_combined.tif 220813_Lgl1451R1An4w1_combined.tif 220813_Lgl1451R1An4w2_combined.tif 230813_Lgl1451R1An1w1_combined.tif 230813_Lgl1451R1An1w2_combined.tif 260613_An11_Lgl_1451R1_w_combined.tif 300713_An1_Lgl_1451R1_w_combined.tif